| S. No. | Topic | Hands-on Experiments and Theories |
| 1. | Introduction to Laboratory | Lab organization, Biosafety, Quality Control |
| 2. | Cell Types and Characteristics | Types of cells: primary, secondary, continuous cell lines; adherent vs. suspension cultures. |
| 3. | Introduction to Cell Culture, Equipment and Buffers Preparation | Overview of cell culture, history, applications, safety, preparation of buffers, and sterilization techniques. Autoclaving and filtration |
| 4. | Extraction of Trypsin | Animal Tissue Preparation, Tissue Homogenization, Purification, Activation of Trypsinogen and Storage |
| 5. | Basics of Animal Cell Cultures, Animal Cell Culture Media Preparation | Minimum Essential Medium (MEM) Preparation |
| 6. | Trypsin Preparation for Cell Culture | Trypsin Preparation for Cell Culture Applications |
| 7. | Primary Cell Culture (Tissue Explant Culture) | Dissection, Tissue Disaggregation, Enzymatic Digestion, Plating, Incubation |
| 8. | Cell Counting and Viability Assays | Protocol for Manual (trypan blue) and automated methods for counting and viability (hemocytometer). |
