COURSE OBJECTIVES:
Understand the Fundamentals of CRISPR-Cas9 Technology: Understanding the molecular mechanism behind CRISPR-Cas9, including the role of guide RNA, Cas9 nuclease, and the DNA repair mechanisms involved in gene editing.
Explore the Applications of CRISPR-Cas9: Identify the various applications of CRISPR-Cas9 in research, agriculture, medicine, and biotechnology, including gene knockouts, gene therapy, and disease modeling.
Examine Ethical Considerations: Know about the ethical implications of gene editing, including concerns about germline editing, designer babies, and the potential for misuse.
Design and Implement CRISPR Experiments: Learn how to design CRISPR experiments, including designing guide RNAs, selecting appropriate target sequences, and optimizing conditions for efficient gene editing.
Design and Implement CRISPR Experiments: Learn how to design CRISPR experiments, including designing guide RNAs, selecting appropriate target sequences, and optimizing conditions for efficient gene editing.
Analyze CRISPR-Cas9 in Disease Research: Investigate how CRISPR is being used to study genetic diseases, develop therapeutic interventions, and evaluate its potential in personalized medicine.
Understand CRISPR-related Risks and Limitations: Identify potential off-target effects, challenges in precision, and the limitations of CRISPR technology in different biological systems.
Stay Updated on CRISPR Advancements: Track and interpret recent advancements and emerging technologies related to CRISPR, such as CRISPR-based diagnostics, base editing, and epigenome editing.
Develop Practical Skills in CRISPR Techniques: Gain experience in designing and conducting CRISPR-Cas9 experiments, including cloning, transfection, and analysis of gene-editing outcomes.