Cell Culture Monitoring and Maintenance |
S. No. |
Topic |
Hands-on Experiments and Theories |
1. |
Troubleshooting in common cell culture |
Troubleshooting common cell culture problems (e.g., cell clumping, contamination, poor growth). |
2. |
Freezing and thawing of cell lines |
Freezing and thawing of cell lines, ensuring preservation and recovery. |
3. |
Genomic DNA Isolation from Cancerous Tissue Biopsies |
Decontamination, Lysis, Column separation and Elution |
4. |
Agarose Gel Electrophoresis |
Genomic DNA Qualitative analysis |
5. |
Cancer Gene Biomarkers Target for Cancer Cell and Tissues |
PCR Programming for Cancer Gene Target |
6. |
Total Protein Extraction from healthy and cancerous tissues |
Qualitative and Quantitative Analysis Comparative Analysis |