| Cell Culture Monitoring and Maintenance | ||
| S. No. | Topic | Hands-on Experiments and Theories |
| 1. | Cryopreservation and Storage | Introduction to cryopreservation of animal cells and proper storage protocols in liquid nitrogen. Impact of freezing on cell viability and recovery strategies. |
| 2. | Cell Culture Contamination and How to Prevent It | Identifying bacterial, fungal, and mycoplasma contamination in cultures. Introduction to the use of antibiotics in culture media and their proper use |
| 3. | Cell Passaging (Subculturing) in Animal Cell Culture | Cell culture medium (fresh and pre-warmed, if necessary). Preparation of Materials Counting and Dilution of Cells Incubation and Monitoring of Cells Recording the Passage Number |
| 4. | Self-Preparation of different Cell Lines from different tissues | Primary Cell Culture Preparation from Fibroblasts, Hepatocytes |
